DMA Amplification and Tumorigenicity of the Human Melanoma Cell Line MeWo1

نویسندگان

  • Inna Shtromas
  • Bradley N. White
  • Jeanette J. A. Holden
  • Dorothy L. Reimer
  • John C. C. Roder
چکیده

Homogeneously staining regions (HSRs) were found in hypodiploid cells (40%) of a subline of the human melanoma cell line, MeWo, (MeWo-C) but were absent from the hypotetraploid cells (60%). Another subline (MeWo-B) was also shown to contain two populations of cells, 70% hypodiploid and 30% hypotetra ploid. None of the MeWo-B cells contained HSRs, but all four cell types from both sublines shared marker chromosomes indi cating their common origin. The hypodiploid MeWo-B cells were karyotypically similar to the hypodiploid MeWo-C cells except for the presence of the HSRs in the latter. Both MeWo-C and MeWoB sublines were injected into BALB/c nude mice. The MeWo-C cells were markedly more tumorigenic than MeWo-B cells as judged by tumor incidence, latency, average tumor size, and tumor take values. Cytogenetic and flow cytofluorometric anal yses of the tumors induced by MeWo-C cells revealed a shift in the tumor cell population from 40% to >90% HSR-containing hypodiploid cells during tumor growth. Hybridization of tumor DNA to a probe (D15Z1), the sequence of which is amplified in the HSRs, also indicated an increase in the proportion of HSRbearing cells during tumor growth. No such selective advantage was found with the hypodiploid, HSR-lacking MeWo-B cells. The results suggest that HSRs found in the human melanoma line MeWo may confer enhanced tumorigenicity to the cells contain ing them.

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تاریخ انتشار 2006